Rapid Cell Magnetisation Using Cationised Magnetoferritin
暫譯: 快速細胞磁化技術:使用陽離子化磁性鐵蛋白
Correia Carreira, Sara
- 出版商: Springer
- 出版日期: 2018-08-04
- 售價: $4,600
- 貴賓價: 9.5 折 $4,370
- 語言: 英文
- 頁數: 166
- 裝訂: Quality Paper - also called trade paper
- ISBN: 3319868381
- ISBN-13: 9783319868387
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商品描述
Bringing together nanoscience with stem cell and bacterial cell biology, this thesis is truly interdisciplinary in scope. It shows that the creation of superparamagnetic nanoparticles inside a protein coat, followed by chemical functionalisation of the protein surface, provides a novel methodology for cell magnetisation using incubation times as short as one minute. Crucially, stem cell proliferation and multi-lineage differentiation capacity is not impaired after labelling. Due to the unspecific labelling mechanism, this thesis also shows that the same magnetic protein nanoparticles can be used for rapid bacterial magnetisation. Thus, it is possible to magnetically capture and concentrate pathogens from clinical samples quickly and highly efficiently.
商品描述(中文翻譯)
本論文將奈米科學與幹細胞及細菌細胞生物學結合,具有真正的跨學科範疇。研究顯示,在蛋白質外殼內創建超順磁性奈米顆粒,隨後對蛋白質表面進行化學功能化,提供了一種新穎的方法論,用於細胞磁化,孵育時間短至一分鐘。關鍵是,幹細胞的增殖和多系統分化能力在標記後並未受到損害。由於非特異性標記機制,本論文還顯示相同的磁性蛋白奈米顆粒可以用於快速的細菌磁化。因此,可以快速且高效地從臨床樣本中磁性捕獲和濃縮病原體。